Rainbow Kaposi's Sarcoma-Associated Herpesvirus Revealed Heterogenic Replication with Dynamic Gene Expression
Ken-ichi Nakajima, Sara Guevara-Plunkett, Frank Y.S. Chuang, Kang-Hsin Wang, Yuanzhi Lyu, Ashish Kumar, Guillaume Luxardi, Chie Izumiya, Athena M. Soulika, Mel Campbell, Yoshihiro Izumiya
Abstract
Sensitivity and resolution of molecular analysis are often compromised by the use of techniques that measure the ensemble average of large cell populations. Having a research tool to nondestructively identify the KSHV replication stage in an infected cell would not only allow us to effectively isolate cells of interest from cell populations but also enable more precise sample selection for advanced single-cell analysis. We prepared a recombinant KSHV that can report on its replication stage in host cells by differential fluorescence emission. Consistent with previous host gene expression studies, our experiments reveal the highly heterogenic nature of KSHV replication/gene expression at individual cell levels. The utilization of a newly developed reporter-KSHV and initial characterization of KSHV replication in single cells are presented.