Acetate and Acetyl-CoA Metabolism of ANME-2 Anaerobic Archaeal Methanotrophs
Heleen T. Ouboter, Arslan Arshad, Stefanie Berger, Jesus Gerardo Saucedo Sanchez, Huub J. M. Op den Camp, Mike S. M. Jetten, Cornelia U. Welte, Julia M. Kurth
Abstract
Acetyl-CoA plays a key role in carbon metabolism and is found at the junction of many anabolic and catabolic reactions. This work describes the biochemical properties of ACS and ACD enzymes from ANME-2 archaea. This adds to our knowledge of archaeal ACS and ACD enzymes, only a few of which have been characterized to date. Furthermore, we validated the in situ activity of ACS in ANME-2d, showing the conversion of acetate into methane by an enrichment culture dominated by ANME-2d.
Topics & Concepts
ArchaeaCatabolismEnzymeMetabolismAnabolismEuryarchaeotaBiochemistryAnaerobic exerciseThermophileBiologyMicrobial metabolismChemistryMethaneBacteriaEnvironmental chemistryEcologyPhysiologyGeneGeneticsMethane Hydrates and Related PhenomenaAnaerobic Digestion and Biogas ProductionMicrobial bioremediation and biosurfactants