<i>TEMPy</i>2: a Python library with improved 3D electron microscopy density-fitting and validation workflows
Tristan Cragnolini, Harpal Sahota, Agnel Praveen Joseph, Aaron Sweeney, Sony Malhotra, Daven Vasishtan, Maya Topf
Abstract
Structural determination of molecular complexes by cryo-EM requires large, often complex processing of the image data that are initially obtained. Here, TEMPy2, an update of the TEMPy package to process, optimize and assess cryo-EM maps and the structures fitted to them, is described. New optimization routines, comprehensive automated checks and workflows to perform these tasks are described.
Topics & Concepts
Python (programming language)WorkflowComputer scienceComputer graphics (images)DatabaseProgramming languageAdvanced Electron Microscopy Techniques and ApplicationsElectron and X-Ray Spectroscopy TechniquesForce Microscopy Techniques and Applications