Transcriptome profiling and protease inhibition experiments identify proteases that activate H3N2 influenza A and influenza B viruses in murine airways
Anne Harbig, Marco Mernberger, Linda Bittel, Stephan Pleschka, Klaus Schughart, Torsten Steinmetzer, Thorsten Stiewe, Andrea Nist, Eva Böttcher‐Friebertshäuser
Abstract
Our results indicate that TMPRSS4 is an IBV-activating protease in murine AECIIs and suggest that TMPRSS13, hepsin, and prostasin cleave H3 and IBV HA in mice. They further show that hepsin and prostasin orthologs might contribute to the differences observed in TMPRSS2-independent activation of H3 in murine and human airways.
Topics & Concepts
ProteasesTranscriptomeProteaseVirologyBiologyMicrobiologyProtease inhibitor (pharmacology)Gene expression profilingEnzymeGeneVirusGene expressionBiochemistryViral loadAntiretroviral therapyInfluenza Virus Research StudiesRespiratory viral infections researchinterferon and immune responses