A bipedal DNA nanowalker fueled by catalytic assembly for imaging of base-excision repairing in living cells
Meng-Mei Lv, Jinwen Liu, Ru‐Qin Yu, Jian‐Hui Jiang
Abstract
catalyzed hairpin assembly (CHA). Our nanomachine outperforms the conventional single leg-based DNA walker with an improved sensitivity, kinetics and walking steps. Moreover, in contrast to the single leg-based DNA walker, the bipedal DNA walker is capable of monitoring the fluorescence signal of reduced APE1 activity, thus indicating amplified intracellular imaging. This bipedal DNA-propelled DNA walker presents a simple and modular amplification mechanism for intracellular biomarkers of interest, providing an invaluable platform for low-abundance biomarker discovery leading to the accurate identification and effective treatment of cancers.
Topics & Concepts
DNABase (topology)IntracellularFluorescenceKineticsChemistryBiophysicsBase excision repairSensitivity (control systems)Base pairFluorescence-lifetime imaging microscopyDNA damageBiologyBiochemistryPhysicsEngineeringOpticsMathematical analysisQuantum mechanicsElectronic engineeringMathematicsAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryDNA and Nucleic Acid Chemistry