Analysis of Cryptococcal Extracellular Vesicles: Experimental Approaches for Studying Their Diversity Among Multiple Isolates, Kinetics of Production, Methods of Separation, and Detection in Cultures of Titan Cells
Flavia C. G. Reis, Bianca Gimenez, Luísa J. Jozefowicz, Rafael F. Castelli, Sharon T. Martins, Lysangela R. Alves, Haroldo César de Oliveira, Márcio L. Rodrigues
Abstract
Fungal extracellular vesicles (EVs) are considered to be important players in the biology of fungal pathogens. However, the limitations in the methodological approaches to studying fungal EVs impair the expansion of knowledge in this field. In the present study, we used the Cryptococcus genus as a model for the study of EVs. We explored the simplification of protocols for EV analysis, which helped us to address some important, but still unanswered, questions about fungal EVs.
Topics & Concepts
Extracellular vesiclesKineticsBiologyExtracellularChemistryComputational biologyCell biologyQuantum mechanicsPhysicsExtracellular vesicles in diseaseFungal Infections and StudiesStudies on Chitinases and Chitosanases