Plasmid hypermutation using a targeted artificial DNA replisome
Xiao Yi, Joleen Khey, Romas J. Kazlauskas, Michael Travisano
Abstract
-fold with only a 78-fold increase in off-target mutagenesis. It was used to evolve itself to increase error rate and increase the efficiency of an efflux pump while simultaneously expanding the substrate repertoire. TADR enables multiple simultaneous substitutions to discover functions inaccessible by accumulating single substitutions, affording potential for solving hard problems in molecular evolution and developing biologic drugs and industrial catalysts.
Topics & Concepts
ReplisomePlasmidSomatic hypermutationBiologyDNAGeneticsDNA replicationCell biologyComputational biologyIn vivoCircular bacterial chromosomeB cellAntibodyBacteriophages and microbial interactionsBacterial Genetics and BiotechnologyAntibiotic Resistance in Bacteria