Kinetic and thermodynamic analysis defines roles for two metal ions in DNA polymerase specificity and catalysis
Shanzhong Gong, Serdal Kırmızıaltın, Adrienne Chang, Joshua E. Mayfield, Yan Zhang, Kenneth A. Johnson
Abstract
. Molecular dynamics simulations also revealed the importance of the divalent cation cloud surrounding exposed phosphates on the DNA. These results enlighten the roles of the two metal ions in the specificity of DNA polymerases.
Topics & Concepts
DNAMetal ions in aqueous solutionDNA polymeraseChemistryCatalysisPolymeraseMetalIonBiochemistryGeneticsBiologyOrganic chemistryDNA and Nucleic Acid ChemistryMetal complexes synthesis and propertiesRNA and protein synthesis mechanisms