Mitochondrial respiration supports autophagy to provide stress resistance during quiescence
Silvia Magalhaes Novais, Jan Blecha, Ravindra Naraine, Jana Mikešová, Pavel Abaffy, Alena Pecinová, Mirko Milošević, Romana Bohuslavová, Jan Procházka, Shawez Khan, Eliška Novotná, Radek Šindelka, Radek Macháň, Mieke Dewerchin, Erik Vlčák, Joanna Kalucka, Soňa Hubáčková, Aleš Benda, Jermaine Goveia, Tomáš Mráček, Cyril Bařinka, Peter Carmeliet, Jiřı́ Neužil, Kateřina Rohlenová, Jakub Rohlena
Abstract
; CI, respiratory complexes I; DCF-DA: 2',7'-dichlordihydrofluorescein diacetate; DHE: dihydroethidium; DSS: dextran sodium sulfate; ΔΨmi: mitochondrial inner membrane potential; EdU: 5-ethynyl-2'-deoxyuridine; ETC: electron transport chain; FA: formaldehyde; HUVEC; human umbilical cord endothelial cells; IBD: inflammatory bowel disease; LC3B: microtubule associated protein 1 light chain 3 beta; LPS: lipopolysaccharide; MEFs: mouse embryonic fibroblasts; MTORC1: mechanistic target of rapamycin kinase complex 1; mtDNA: mitochondrial DNA; NAC: N-acetyl cysteine; OXPHOS: oxidative phosphorylation; PCs: proliferating cells; PE: phosphatidylethanolamine; PEITC: phenethyl isothiocyanate; QCs: quiescent cells; ROS: reactive oxygen species; PLA2: phospholipase A2, WB: western blot.