Investigating Anti-Cancer Activity of Dual-Loaded Liposomes with Thymoquinone and Vitamin C
Ahmed Munef, Zainab Lafi, Naeem Shalan
Abstract
Background: Thymoquinone (TQ) and vitamin C (Vit C) have demonstrated individual anticancer effects in various studies. TQ exhibits inhibitory properties against tumor growth, induces apoptosis, while Vit C protects against DNA damage and oxidative stress.Aim: Formulation of TQ and Vit C combination into liposomes using two methods and investigate the synergistic anticancer.Method: Liposomal preparations were characterized, and the purity of drug components was confirmed using encapsulation efficiency (EE %). Results:In vitro cell viability studies demonstrated the inhibitory effect of TQ and Vit C against colorectal (HT29, 5.5 ± 0.9 μM) and lung cancer (A549, 6.25 ± 0.9 μM) cell lines with combination index <1.Conclusion: The formulation of TQ and Vit C displayed synergistic anticancer activity.