High Glucose Induces Lipid Accumulation via 25-Hydroxycholesterol DNA-CpG Methylation
Yaping Wang, Lanming Chen, William M. Pandak, Douglas M. Heuman, Phillip B. Hylemon, Shunlin Ren
Abstract
CpG levels in at least 2,225 genes involved in 57 signaling pathways. The hypermethylated genes directly involved in carbohydrate and lipid metabolism are of PI3K, cAMP, insulin, insulin secretion, diabetic, and NAFLD signaling pathways. The studies indicate a close relationship between the increase in nuclear 25HC levels and activation of DNMT1, which may regulate lipid metabolism via DNA CpG methylation. Our results indicate an epigenetic regulation of hepatic cell metabolism that has relevance to some common diseases such as non-alcoholic fatty liver disease and metabolic syndrome.
Topics & Concepts
DNA methylationLipid metabolismEpigeneticsDNMT1BiologyMethylationCarbohydrate metabolismFatty liverCpG siteDNA methyltransferaseBiochemistryCell biologyChemistryGene expressionGeneInternal medicineDiseaseMedicineCholesterol and Lipid MetabolismLipid metabolism and biosynthesisDiet, Metabolism, and Disease