Virtual histopathology with ultraviolet scattering and photoacoustic remote sensing microscopy
Nathaniel J. M. Haven, Matthew T. Martell, Brendyn D. Cikaluk, Brendon S. Restall, E. D. McAlister, Sveta Silverman, Lashan Peiris, Jean Deschênes, Xingyu Li, Roger J. Zemp
Abstract
Realistic label-free virtual histopathology has been a long sought-after goal not yet achieved with current methods. Here, we introduce high-resolution hematoxylin and eosin (H&E)-like virtual histology of unstained human breast lumpectomy specimen sections using ultraviolet scattering-augmented photoacoustic remote sensing microscopy. Together with a colormap-matching algorithm based on blind stain separation from a reference true H&E image, we are able to produce virtual H&E images of unstained tissues with close concordance to true H&E-stained sections, with promising diagnostic utility.