Protected <i>N</i>-Acetyl Muramic Acid Probes Improve Bacterial Peptidoglycan Incorporation via Metabolic Labeling
Ashley R. Brown, Kimberly A. Wodzanowski, Cintia C. Santiago, Stephen N. Hyland, Julianna L. Follmar, PapaNii Asare-Okai, Catherine L. Grimes
Abstract
do not utilize peracetylated peptidoglycan substrates but do employ methyl esters. The 10-fold improvement of probe utilization indicates that (i) masking the carboxylic acid is favorable for transport and (ii) bacterial esterases are capable of removing the methyl ester for use in peptidoglycan biosynthesis. This investigation advances bacterial cell wall biology, offering a prescription on how to best deliver and utilize bacterial metabolic glycan probes.
Topics & Concepts
PeptidoglycanGlycanBacterial cell structureBiochemistryCell envelopeCell wallGlycobiologyMuramic acidBiologyBacteriaComputational biologyChemistryEscherichia coliGlycoproteinGeneGeneticsGlycosylation and Glycoproteins ResearchBacterial Genetics and BiotechnologyCarbohydrate Chemistry and Synthesis