Employing Label‐free Electrochemical Biosensor Based on 3D‐Reduced Graphene Oxide and Polyaniline Nanofibers for Ultrasensitive Detection of Breast Cancer BRCA1 Biomarker
Ya‐Mu Xia, Mengying Li, Chenglong Chen, Meng Xia, Wen Zhang, Wei‐Wei Gao
Abstract
Abstract A label‐free DNA biosensor based on three‐dimensional reduced graphene oxide (3D‐rGO) and polyaniline (PANI) nanofibers modified glassy carbon electrode (GCE) was successfully developed for supersensitive detection of breast cancer BRCA1. The results demonstrated that 3D‐rGO and PANI nanofibers had synergic effects for reducing the charge transfer resistance ( R ct ), meaning a huge enhancement in electrochemical activity of 3D‐rGO‐PANI/GCE. Probe DNA could be immobilized on 3D‐rGO‐PANI/GCE for special and sensitive recognition of target DNA (1.0×10 −15 –1.0×10 −7 M) with a theoretical LOD of 3.01×10 −16 M (3 S/m ). Furthermore, this proposed nano‐biosensor could directly detect BRCA1 in real blood samples.