Chemically modified small interfering RNA targeting Hedgehog signaling pathway for rheumatoid arthritis therapy
Lang Lin, Shangling Zhu, Hongyu Huang, Lin-Ping Wu, Jianlin Huang
Abstract
gene. With precise chemical modifications, siRNAs' efficacy and stability are significantly improved, and the off-target effects are minimized. The optimized chemically modified siRNA (si-S1A3-Chol) decreases RA-FLS proliferation and invasiveness without the transfection reagent. Furthermore, si-S1A3-Chol injected intra-articularly effectively alleviates joint destruction and improves motor function in collagen-induced arthritis mouse models. Consequently, our results demonstrate that chemically modified siRNA targeting the Hedgehog signaling pathway may be a potential therapy for RA.
Topics & Concepts
SmoothenedSmall interfering RNAHedgehog signaling pathwayHedgehogSignal transductionCancer researchRheumatoid arthritisSynovitisRNA interferenceCell biologyArthritisChemistryMedicineBiologyTransfectionImmunologyRNAGeneBiochemistryHedgehog Signaling Pathway StudiesChromatin Remodeling and CancerTumors and Oncological Cases