An NADH-selective and sensitive fluorescence probe to evaluate living cell hypoxic stress
Mingzhe Li, Chang Liu, Wenjuan Zhang, Longfei Xu, Miaomiao Yang, Zhaoli Chen, Xinxing Wang, Lingling Pu, Weili Liu, Xianshun Zeng, Tianhui Wang
Abstract
cannot be oxidized by the mitochondrial electron transport chain, which leads to the accumulation of reducing equivalents and subsequent reduction stress. Detecting changes in intracellular NADH levels is expected to allow an assessment of stress. We synthesized a red fluorescent probe, DPMQL1, with high selectivity and sensitivity for detecting NADH in living cells. The probe DPMQL1 has strong anti-interference abilities toward various potential biological interferences, such as metal ions, anions, redox species, and other biomolecules. In addition, its detection limit can reach the nanomolar level, meaning it can display small changes in NADH levels in living cells, so as to realize the evaluation of cell-based hypoxic stress.