Optimization of Peripheral Blood Mononuclear Cell Extraction from Small Volume of Blood Samples: Potential Implications for Children-Related Diseases
Deanira Patrone, Nicola Alessio, Nicola Antonucci, Anna Lisa Brigida, Gianfranco Peluso, Umberto Galderisi, Dario Siniscalco
Abstract
Managing medical procedures for children with problematic disorders is a challenging approach, especially in the case of blood withdrawal for autism spectrum disorder-affected children. Peripheral blood mononuclear cells (PBMC) represent an important cellular model to study immune responses and drug toxicity. The monocytic cells, a fraction of PBMC, are strongly involved in some pathophysiological processes, such as inflammation and immune system changes. Here, we propose a simple, reliable protocol for obtaining peripheral blood-derived mononuclear cells from small volumes of blood samples.
Topics & Concepts
Peripheral blood mononuclear cellImmune systemImmunologyMedicinePeripheral bloodInflammationPeripheralPathophysiologyBiologyPathologyInternal medicineIn vitroBiochemistryPancreatic function and diabetesDiabetes and associated disordersImmune Cell Function and Interaction