Alicyclobacillus mali sp. nov., Alicyclobacillus suci sp. nov. and Alicyclobacillus fructus sp. nov., thermoacidophilic sporeforming bacteria isolated from fruit beverages
Katerina Roth, Yadwinder Singh Rana, Devin Daeschel, Jasna Kovač, Randy W. Worobo, Abigail B. Snyder
Abstract
Six thermo-acidophilic, spore-forming strains were isolated from a variety of juice products and were characterized genetically and phenotypically. According to 16S rRNA and rpoB gene phylogenetic analyses and average nucleotide identity comparisons against the species demarcation cutoff at <95 %, these six strains were determined to represent three novel species of Alicyclobacillus . The isolates were designated FSL-W10-0018 T , FSL-W10-0037, FSL-W10-0048, VF-FSL-W10-0049 T , FSL-W10-0057 and FSL-W10-0059 T . All six isolates were Gram-positive, motile, rod shaped, contained menaquinone 7 as the major respiratory quinone and had ω-cyclohexane C 17 : 0 as a major fatty acid. They were all able to grow aerobically in a range of acidic and moderate thermal conditions. Only isolates FSL-W10-0048 and VF-FSL-W10-0049 T were able to produce guaiacol. The following names are proposed for the three new species: Alicyclobacillus mali sp. nov. (type strain FSL-W10-0018 T = DSM 112016 T =NCIMB 15266 T ); Alicyclobacillus suci sp. nov (VF-FSL-W10-0049 T =DSM 112017 T =NCIMB 15265 T ); and Alicyclobacillus fructus sp. nov. (FSL-W10-0059 T =DSM 112018 T =NCIMB 15264 T ).