Split-luciferase Complementation Imaging Assay to Study Protein-protein Interactions in Nicotiana benthamiana
Liping Wang, Gang Yu, Alberto Macho, Rosa Lozano-Durán
Abstract
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Topics & Concepts
Protein-fragment complementation assayLuciferaseComplementationNicotiana benthamianaBimolecular fluorescence complementationYeastProtein–protein interactionChemistryComputational biologyCell biologyFluorescence-lifetime imaging microscopyBiochemistrySaccharomyces cerevisiaeBiologyEnzymeIdentification (biology)FluorescenceBiophysicsFluorescent proteinSubcellular localizationSensitivity (control systems)Molecular biologyPreclinical imagingTransgenic Plants and ApplicationsPlant Virus Research StudiesToxin Mechanisms and Immunotoxins