An ultrasensitive electrochemical biosensor for microRNA-21 detection <i>via</i> AuNPs/GAs and Y-shaped DNA dual-signal amplification strategy
Congjuan He, Jiaying Zhao, Yanyi Long, Huisi Yang, Jiangbo Dong, Huan Liu, Zhikun Hu, Mei Yang, Danqun Huo, Changjun Hou
Abstract
Herein, a gold nanoparticles/graphene aerogels (AuNPs/GAs) modified electrochemical biosensor with catalytic hairpin assembly (CHA) and Y-shaped DNA nanostructure dual-signal amplification approaches for ultrasensitive microRNA-21 (miR-21) detection was successfully constructed, which displayed an ultra-wide detection linear range from 5 fM to 50 nM, as well as a relatively low detection limit (LOD) of 14.70 aM (S/N = 3). Furthermore, the sensing strategy had excellent specificity among highly homologous miRNA family members and exhibited satisfactory analytical performance for miRNA detection.
Topics & Concepts
Detection limitColloidal goldBiosensorLinear rangeDNARolling circle replicationNanotechnologyMaterials scienceNanostructureCombinatorial chemistryNanoparticleChemistryChromatographyPolymeraseBiochemistryAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryMicroRNA in disease regulation