Leader peptide exchange to produce hybrid, new-to-nature ribosomal natural products
Laura Franz, Jesko Koehnke
Abstract
Ribosomal natural products contain exquisite post-translational peptide modifications that are installed by a range of pathway-specific enzymes. We present proof of principle for a Sortase A-based approach that enables peptide modification by enzymes from unrelated pathways. This allowed the one-pot synthesis of a new-to-nature, hybrid ribosomal natural product.
Topics & Concepts
PeptideSortase ASortaseChemistryRibosomal RNAEnzymeComputational biologyPeptide BiosynthesisNatural (archaeology)BiochemistryCombinatorial chemistryBiologyBacterial proteinRibosomeGeneRNAPaleontologyBiochemical and Structural CharacterizationMicrobial Natural Products and BiosynthesisMachine Learning in Bioinformatics