Lifestyle adaptations of <i>Rhizobium</i> from rhizosphere to symbiosis
Rachel M. Wheatley, Brandon Ford, Li Li, Samuel T. N. Aroney, Hayley E. Knights, Raphael Ledermann, Alison K. East, Vinoy K. Ramachandran, Philip S. Poole
Abstract
Significance Rhizobia are soil-dwelling bacteria that form symbioses with legumes and provide biologically useable nitrogen as ammonium for the host plant. High-throughput DNA sequencing has led to a rapid expansion in publication of complete genomes for numerous rhizobia, but analysis of gene function increasingly lags gene discovery. Mariner-based transposon insertion sequencing has allowed us to characterize the fitness contribution of bacterial genes and determine those functionally important in a Rhizobium– legume symbiosis at multiple stages of development.
Topics & Concepts
RhizobiaBiologyRhizobiumSymbiosisRhizosphereTransposable elementNitrogen fixationGeneGenomeFunction (biology)DNA sequencingGeneticsComputational biologyBacteriaLegume Nitrogen Fixing SymbiosisNematode management and characterization studiesAgronomic Practices and Intercropping Systems