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Enzymes Immobilized into Starch- and Gelatin-Based Hydrogels: Properties and Application in Inhibition Assay

Elena N. Esimbekova, Irina G. Torgashina, Elena V. Nemtseva, Valentina A. Kratasyuk

2023Micromachines18 citationsDOIOpen Access PDF

Abstract

The present work is a review of the research on using hydrogels based on natural biodegradable polymers, starch, and gelatin for enzyme immobilization. This review addresses the main properties of starch and gelatin that make them promising materials in biotechnology for producing enzyme preparations stable during use and storage and insensitive to chemical and physical impacts. The authors summarize their achievements in developing the preparations of enzymes immobilized in starch and gelatin gels and assess their activity, stability, and sensitivity for use as biorecognition elements of enzyme inhibition-based biosensors.

Topics & Concepts

GelatinSelf-healing hydrogelsStarchBiosensorEnzymeNatural polymersImmobilized enzymeChemistryPolymerEnzyme assayBiochemistryMaterials sciencePolymer chemistryOrganic chemistryEnzyme Catalysis and ImmobilizationElectrochemical sensors and biosensorsProtein Hydrolysis and Bioactive Peptides
Enzymes Immobilized into Starch- and Gelatin-Based Hydrogels: Properties and Application in Inhibition Assay | Litcius