deepBlink: threshold-independent detection and localization of diffraction-limited spots
Bastian Eichenberger, Yinxiu Zhan, Markus Rempfler, Luca Giorgetti, Jeffrey A. Chao
Abstract
Detection of diffraction-limited spots in single-molecule microscopy images is traditionally performed with mathematical operators designed for idealized spots. This process requires manual tuning of parameters that is time-consuming and not always reliable. We have developed deepBlink, a neural network-based method to detect and localize spots automatically. We demonstrate that deepBlink outperforms other state-of-the-art methods across six publicly available datasets containing synthetic and experimental data.
Topics & Concepts
SpotsBiologyDiffractionPattern recognition (psychology)Artificial intelligenceBiological systemProcess (computing)Computer scienceOpticsPhysicsOperating systemBotanyCell Image Analysis TechniquesAdvanced Electron Microscopy Techniques and ApplicationsAdvanced Fluorescence Microscopy Techniques