Study of Alveolar Bone Remodeling Using Deciduous Tooth Stem Cells and Hydroxyapatite by Vascular Endothelial Growth Factor Enhancement and Inhibition of Matrix Metalloproteinase-8 Expression in vivo

Abstract

Background: Periodontitis progression is characterized by alveolar bone loss, and its prevention is a major clinical problem in
\nperiodontal disease management. Matrix metalloproteinase-8 (MMP-8) has been shown to adequately monitor the treatment of chronic
\nperiodontitis patients as gingival crevicular fluid MMP-8s were positively associated with the severity of periodontal disease.
\nMoreover, modulating the vascular endothelial growth factor (VEGF) levels in bones could be a good way to improve bone
\nregeneration and cure periodontitis as VEGF promotes endothelial cell proliferation, proteolytic enzyme release, chemotaxis, and
\nmigration; all of which are required for angiogenesis.
\nPurpose: The aim of this study was to determine the effect of hydroxyapatite incorporated with stem cells from exfoliated deciduous
\nteeth (SHED) in Wistar rats’ initial alveolar bone remodeling based on the findings of MMP-8 and VEGF expressions.
\nMethods: A hydroxyapatite scaffold (HAS) in conjunction with SHED was transplanted into animal models with alveolar mandibular
\ndefects. A total of 10 Wistar rats (Rattus norvegicus) were divided into two groups: HAS and HAS + SHED. Immunohistochemistry
\nstaining was performed after 7 days to facilitate the examination of MMP-8 and VEGF expressions.
\nResults: The independent t-test found significant downregulation of MMP-8 and upregulation VEGF expressions in groups
\ntransplanted with HAS in conjunction with SHED compared with the HAS group (p < 0.05).
\nConclusion: The combination of SHED with HAS on alveolar bone defects may contribute to initial alveolar bone remodeling as
\nevident through the assessments of MMP-8 and VEGF expressions.

Topics & Concepts