A Shared Perspective on <i>in Vitro</i> and <i>in Vivo</i> Models to Assay Intestinal Transepithelial Transport of Food Compounds
Arancha Hevia, Patricia Ruas‐Madiedo, Miguel A. Faria, Valérie Petit, Bruna Gomes Alves, Paula Alvito, Elena Arranz, Shanna Bastiaan‐Net, Milena Corredig, Wieneke Dijk, Didier Dupont, Linda Giblin, Brigitte A. Graf, Alina Kondrashina, Helena Ramos, Lorena Ruíz, Marta Santos-Hernández, Laura Soriano-Romaní, Lidia Tomás‐Cobos, Santiaga María Vivanco-Maroto, Isidra Recio, Beatriz Miralles
Abstract
Assessing nutrient bioavailability is complex, as the process involves multiple digestion steps, several cellular environments, and regulatory-metabolic mechanisms. Several in vitro models of different physiological relevance are used to study nutrient absorption, providing significant challenges in data evaluation. However, such in vitro models are needed for mechanistic studies as well as to screen for biological functionality of the food structures designed. This collaborative work aims to put into perspective the wide-range of models to assay the permeability of food compounds considering the particular nature of the different molecules, and, where possible, in vivo data are provided for comparison.