MicroRNA-219 loaded chitosan nanoparticles for treatment of glioblastoma
Rawan Alswailem, Fulwah Yahya Alqahtani, Fadilah Sfouq Aleanizy, Bahauddeen M. Alrfaei, Mohammad Badran, Qamraa H. Alqahtani, Hosam Gharib Abdelhady, Ibrahim A. Alsarra
Abstract
release study demonstrated sustained release pattern of miR-219 from CS-NPs. Gel retardation assay has confirmed the integrity of miR-219 after production process. The fabricated NPs reduced the survival of U87 MG cells to 78% after 24 h of post-transfection, and into 67.5% after 48 h. However, fibroblasts were not affected by the NPs, revealing their specificity for GBM cells. Given the tumour suppressing function of miR-219, and advantage of CS-NPs for gene delivery to the central nervous system, the presented NPs have a great potential for treatment of GBM.
Topics & Concepts
ChitosanTransfectionZeta potentialApoptosisGlioblastomaNanoparticleIn vitroDispersitymicroRNAU87Cell cultureMaterials scienceBiophysicsChemistryCancer researchMolecular biologyNanotechnologyMedicineBiochemistryGeneBiologyPolymer chemistryGeneticsRNA Interference and Gene DeliveryMicroRNA in disease regulationAdvanced biosensing and bioanalysis techniques