C-C chemokine receptor type 2-overexpressing exosomes alleviated experimental post-stroke cognitive impairment by enhancing microglia/macrophage M2 polarization
Huaichun Yang, Min Zhang, Rui Wu, Haiqing Zheng, Liying Zhang, Jing Luo, Lili Li, Xiquan Hu
Abstract
BACKGROUND: ) can enhance the therapeutic effects on PSCI and the possible underlying mechanisms have not been studied. AIM: can enhance therapeutic effects on PSCI. METHODS: on PSCI was assessed by Morris water maze. Remyelination and oligodendrogenesis were analyzed by Western blotting and immunofluorescence microscopy. QRT-PCR and immunofluorescence microscopy were conducted to compare the microglia/macrophage polarization. The infiltration and activation of hematogenous macrophages were analyzed by Western blotting and transwell migration analysis. RESULTS: treated group. CONCLUSION: CCR2 over-expression enhanced the therapeutic effects of exosomes on the experimental PSCI by promoting M2 microglia/macrophage polarization, enhancing oligodendrogenesis and remyelination. These therapeutic effects are likely through suppressing the CCL2-induced hematogenous macrophage migration and activation.