A concise review on development of probiotics from Lactobacillus using CRISPR-Cas technology of gene editing
Siddharth Vaid, Vinay Kumar Pandey, Rahul Singh, Aamir Hussain Dar, Rafeeya Shams, Ketan Singh Thakur
Abstract
Immunological nourishment is the most active part of our health system that plays a crucial role in the human body's functioning. With the hectic rush of life running at present time, health management is becoming a concern. The best alternative to health management is to provide the required nutritional elements in a ready-to-consume form and get absorbed in the body without too much time consumption. The use of probiotics has become a popular way to manage digestive and immunological health in recent years. The difficulty of using CRISPR technology to introduce foreign DNA into numerous bacterial species has been overcome by redesigning the CRISPR tools to benefit the requirements of the target species. This review is focused on the study of the CRISPR-Cas system to develop a probiotic by editing the genome of the selected strain of probiotic microorganisms and providing the best alternative for health management in its study.