Mutability landscape guided engineering of a promiscuous microbial glycosyltransferase for regioselective synthesis of salidroside and icariside D2
Guosi Li, Wei Wang, Heng Guo, Shanyong Yi, Fang Wang, Shiping Huang, Nan Hu, Qilin Xu, Yongjun Zang, Bangxing Han, Xinjian Yin
Topics & Concepts
RegioselectivityDirected evolutionSaturated mutagenesisChemistryGlycosyltransferaseMutantSalidrosideStereochemistryBiochemistryEnzymeGeneChromatographyCatalysisMedicinal Plants and Bioactive CompoundsPharmacological Effects of Natural CompoundsPlant biochemistry and biosynthesis