Dynamic network-guided CRISPRi screen identifies CTCF-loop-constrained nonlinear enhancer gene regulatory activity during cell state transitions
Renhe Luo, Jielin Yan, Jin Woo Oh, Xi Wang, Dustin Shigaki, Wilfred Hing Sang Wong, Hyunwoo Cho, Dylan Murphy, Ronald Cutler, Bess P. Rosen, Julián Pulecio, Dapeng Yang, Rachel A. Glenn, Tingxu Chen, Qing V. Li, Thomas Vierbuchen, Simone Sidoli, Effie Apostolou, Danwei Huangfu, M Beer
Topics & Concepts
EnhancerBiologyGene regulatory networkComputational biologyCTCFEnhancer RNAsRegulation of gene expressionEndodermTranscription factorGeneticsGeneCell biologyEmbryonic stem cellGene expressionGenomics and Chromatin DynamicsCRISPR and Genetic EngineeringGene Regulatory Network Analysis