Characterization, enzymatic and biochemical properties of endophytic bacterial strains of the medicinal plant Ajuga turkestanica (Rgl.) Brig (Lamiaceae)
Bakhodir Mamarasulov, Kakhramon Davranov, Mohammad Shah Jahan, Dilfuza Jabborova, Omaima Nasif, Mohammad Javed Ansari, Subhan Danish, Rahul Datta
Abstract
This research work aims to isolate endophytic bacteria from the Ajuga turkestanica plant that have plant growth promoting properties. The microscopic, enzymatic and biochemical properties of the isolated endophytic bacteria are described. Twelve isolates of endophytic bacteria were isolated by standard microbiological methods. Isolated endophytic bacteria were identified by (MALDI-TOF MS). Endophytic bacteria isolated from the plant were detected in MALDI-TOF MS. Bacterial isolates were dominated by endophytic bacteria belonging to the genus Bacillus, Pseudomonas, Siccibacter. When the growth-enhancing properties of cultivated plants used in sustainable agriculture were studied, the isolated bacterial strains showed positive results in siderophor, ammonia, indole acetic acid production, and nitrogen fixation properties. Numerous endophytic bacteria have been found to have the enzymatic activity of oxidase, catalase, amylase, lecithinase and lipase. The results showed that endophytic bacteria isolated from the plant A. turkestanica are have plant stimulating properties which can be shown as a candidate for the production of bioinoculants individually or as part of a consortium.