Solution NMR goes big: Atomic resolution studies of protein components of molecular machines and phase-separated condensates
Alexander I. M. Sever, Rashik Ahmed, Philip Rößler, Lewis E. Kay
Abstract
The tools of structural biology have undergone remarkable advances in the past decade. These include new computational and experimental approaches that have enabled studies at a level of detail - and ease - that were not previously possible. Yet, significant deficiencies in our understanding of biomolecular function remain and new challenges must be overcome to go beyond static pictures towards a description of function in terms of structural dynamics. Solution Nuclear Magnetic Resonance (NMR) spectroscopy has emerged as a powerful technique for atomic resolution studies of the dynamics of a wide range of biomolecules, including molecular machines and the components of phase-separated condensates. Here we highlight some of the very recent advances in these areas that have been driven by NMR.