Heparosan oligosaccharide synthesis using engineered single-function glycosyltransferases
Jian-Qun Deng, Zhen Lü, Juan Liu, Yan Zhao, Xuben Hou, Xue-Ping Guo, Wenjie Jiang, Fengshan Wang, Juzheng Sheng
Abstract
A 24-fold increase in GlcNAc-transferase ability through KfiA screening and engineering. An approach for heparosan oligosaccharide synthesis relying on single-function glycosyltransferases.
Topics & Concepts
GlycosyltransferaseOligosaccharideTransferaseFunction (biology)ChemistryBiochemistryGlycosylationEnzymeBiologyCell biologyGlycosylation and Glycoproteins ResearchProteoglycans and glycosaminoglycans researchCarbohydrate Chemistry and Synthesis