Functional Differences between E. coli and ESKAPE Pathogen GroES/GroEL
Jared Sivinski, Andrew J. Ambrose, Iliya Vitalievich Panfilenko, Christopher J. Zerio, Jason Machulis, Niloufar Mollasalehi, Lynn K. Kaneko, Mckayla Stevens, Anne‐Marie Ray, Yangshin Park, Chunxiang Wu, Quyen Q. Hoang, Steven M. Johnson, Eli Chapman
Abstract
restored organism viability, but produced an elongated phenotype, suggesting differences in chaperonin function, including client specificity and/or refolding cycle rates. These data offer important mechanistic insight into these remarkable machines, and the new strains developed allow for the synthesis of homogeneous chaperonins for biochemical studies and to further our efforts to develop chaperonin-targeted antibiotics.
Topics & Concepts
GroELChaperoninGroESPathogenChemistryMicrobiologyEscherichia coliBiologyComputational biologyVirologyCell biologyProtein foldingBiochemistryGeneHeat shock proteins researchEnzyme Structure and FunctionBacterial Genetics and Biotechnology