Isolation of primary human liver cells from normal and nonalcoholic steatohepatitis livers
Xiao Liu, Kevin Lam, Huayi Zhao, Sadatsugu Sakane, Hyun Young Kim, Álvaro Eguileor, Karin Diggle, Shuai Wu, Raquel Weber, Pejman Soroosh, Mojgan Hosseini, Kristin L. Mekeel, David A. Brenner, Tatiana Kisseleva
Abstract
Here, we present a protocol for isolating human hepatocytes and neural progenitor cells from normal and nonalcoholic steatohepatitis livers. We describe steps for perfusion for scaled-up liver cell isolation and optimization of chemical digestion to achieve maximal yield and cell viability. We then detail a liver cell cryopreservation and potential applications, such as the use of human liver cells as a tool to link experimental and translational research.
Topics & Concepts
Nonalcoholic steatohepatitisProgenitor cellCryopreservationCellHuman liverDigestion (alchemy)Viability assaySteatosisBiologyChemistryPathologyStem cellNonalcoholic fatty liver diseaseMedicineBiochemistryCell biologyInternal medicineFatty liverIn vitroEmbryoChromatographyDiseaseLiver physiology and pathologyPancreatic function and diabetesEndoplasmic Reticulum Stress and Disease