Wastewater surveillance using ddPCR accurately tracked Omicron emergence due to altered N1 probe binding efficiency
Melissa K. Schussman, Adélaïde Roguet, Angela Schmoldt, Brooke Dinan, Sandra L. McLellan
Abstract
Wastewater surveillance for SARS-CoV-2 uses droplet digital (dd)PCR for absolute quantification and high sensitivity. We found ddPCR can also distinguish and accurately quantify Delta and Omicron variants due to a mutation in the probe binding site.
Topics & Concepts
Digital polymerase chain reactionWastewaterChemistryComputer scienceComputational biologyMolecular biologyGeneticsBiologyEnvironmental sciencePolymerase chain reactionEnvironmental engineeringGeneSARS-CoV-2 detection and testingBiosensors and Analytical DetectionAdvanced biosensing and bioanalysis techniques