The small <scp>GTPase</scp> Sar1, control centre of <scp>COPII</scp> trafficking
Sander E. Van der Verren, Giulia Zanetti
Abstract
Sar1 is a small GTPase of the ARF family. Upon exchange of GDP for GTP, Sar1 associates with the endoplasmic reticulum (ER) membrane and recruits COPII components, orchestrating cargo concentration and membrane deformation. Many aspects of the role of Sar1 and regulation of its GTP cycle remain unclear, especially as complexity increases in higher organisms that secrete a wider range of cargoes. This review focusses on the regulation of GTP hydrolysis and its role in coat assembly, as well as the mechanism of Sar1-induced membrane deformation and scission. Finally, we highlight the additional specialisation in higher eukaryotes and the outstanding questions on how Sar1 functions are orchestrated.
Topics & Concepts
COPIIGTPaseCell biologyBiologyEndoplasmic reticulumSecretory pathwayGolgi apparatusCellular transport and secretionPancreatic function and diabetesEndoplasmic Reticulum Stress and Disease