Visualization of multiple localizations of GLUT4 by fluorescent probes of PYP-tag with designed unnatural warhead
Miyako Nishiura, Yuichiro Hori, Maho Umeno, Kazuya Kikuchi
Abstract
-glycosylation of GLUT4 influences intracellular localization. Furthermore, we were able to visually distinguish active GLUT4 molecules that underwent membrane translocation at least twice within an hour from those that remained intracellularly, discovering previously unrecognized dynamic behaviors of GLUT4. This technology provides not only a valuable tool for study on multiple localization and dynamics of proteins but also important information on diseases caused by protein translocation dysfunction.
Topics & Concepts
WarheadVisualizationFluorescenceChemistryComputational biologyComputer scienceBiophysicsBiologyEngineeringArtificial intelligencePhysicsAerospace engineeringOpticsCancer, Hypoxia, and MetabolismMetabolism, Diabetes, and CancerCancer Research and Treatments