A highly selective quinolizinium-based fluorescent probe for cysteine detection
O Wa‐Yi, Wing-Cheung Chan, Caifeng Xu, Jie‐Ren Deng, Ben C.B. Ko, Man‐Kin Wong
Abstract
A novel fluorescent quinolizinium-based turn-off probe has been developed for selective detection of cysteine. The probe showed high selectivity and sensitivity towards cysteine over other amino acids including the similarly structured homocysteine and glutathione with a detection limit of 0.18 μM (S/N = 3). It was successfully applied to cysteine detection in living cells with low cytotoxicity and quantitative analysis of spiked mouse serum samples with moderate to good recovery (96-109%).
Topics & Concepts
CysteineDetection limitGlutathioneFluorescenceChemistryCytotoxicitySelectivityHomocysteineAmino acidChromatographyBiochemistryIn vitroEnzymeQuantum mechanicsPhysicsCatalysisSulfur Compounds in BiologyFolate and B Vitamins ResearchMolecular Sensors and Ion Detection