Listeria monocytogenes GlmR Is an Accessory Uridyltransferase Essential for Cytosolic Survival and Virulence
Daniel A. Pensinger, Kimberly V. Gutierrez, Hans B. Smith, William J. B. Vincent, David S. Stevenson, Katherine A. Black, Krizia M. Perez-Medina, Joseph P. Dillard, Kyu Y. Rhee, Daniel Amador‐Noguez, TuAnh N. Huynh, John‐Demian Sauer
Abstract
mutant is deficient in production of an essential cell-wall metabolite, UDP-GlcNAc, and suppressors that increase metabolite levels also restore virulence. Purified GlmR can directly catalyze the synthesis of UDP-GlcNAc, and this enzymatic activity is conserved in both Bacillus subtilis and Staphylococcus aureus. These results highlight the importance of accessory cell wall metabolism enzymes in responding to cell-wall stress in a variety of Gram-positive bacteria.
Topics & Concepts
VirulenceCytosolListeria monocytogenesTeichoic acidBiologyMicrobiologyMutantCell envelopePathogenCell biologyBacteriaBiochemistryCell wallPeptidoglycanGeneGeneticsEnzymeEscherichia coliListeria monocytogenes in Food SafetyVibrio bacteria research studiesIdentification and Quantification in Food