Simultaneous quantitation of oxidized and reduced glutathione via LC-MS/MS to study the redox state and drug-mediated modulation in cells, worms and animal tissue
Alicia Thiel, Ann-Kathrin Weishaupt, Merle M. Nicolai, Kristina Lossow, Anna P. Kipp, Tanja Schwerdtle, Julia Bornhorst
Topics & Concepts
ChemistryGlutathioneMenadioneChromatographyOxidative stressDetection limitLiquid chromatography–mass spectrometryTandem mass spectrometryGlutathione disulfideIn vivoSample preparationOxidative phosphorylationButhionine sulfoximineMass spectrometryBiochemistryEnzymeBiotechnologyBiologySulfur Compounds in BiologyGlutathione Transferases and PolymorphismsRedox biology and oxidative stress