An overview: LC-ms as tool of sample extraction and quantification in bioanalytical laboratories
K Pramod, Akash Deep, K Pooja, Ajay Singh
Abstract
LC-MS/MS is the hyphenated techniques which is used for the identification and quantification of the organic compounds in bioanalyitical laboratories. LC-MS instrument is basically combination of liquid chromatography unit and mass spectrometer to achieve dual benefits. In other hand, LC-MS/MS is Liquid chromatography with two mass spectrometer units specifically analyzers for better resolution. LC-MS/MS is widely used for separation of biological samples of different origins like protein, urine, blood, saliva, tissue. The prefence are mainly due to its specificity, precision and accuracy. In this review article, we will discuss the use of this hyphenated systems in analysis of protein, oxysterols, stratum corneum and metabonimics. Sample preparation techniques such as protein precipitation, liquid-liquid extraction, and solid phase extraction and affinity techniques and effective analysis by LC/MS/MS. This approach is also preferred for the separation of antibodies, proteins and enzymes from the biological matrix.