Simultaneous Detection of Hypochlorite and Singlet Oxygen by a Thiocoumarin-Based Ratiometric Fluorescent Probe
Moonyeon Cho, Nguyễn Văn Nghĩa, Juyoung Yoon
Abstract
) and the hypochlorite concentrations (a low detection limit of 0.15 μM). Furthermore, upon green light irradiation, Fcoum-S was efficiently desulfurized to its oxo analogue (Fcoum-O) by in situ generated singlet oxygen, leading to a significant change in fluorescence. Fcoum-S could work well in an aqueous medium owing to the high reactivity of the thiocarbonyl group and the aggregation-induced emission characteristics. More importantly, Fcoum-S could target mitochondria and was successfully utilized for fluorescence imaging of mitochondrial hypochlorite/singlet oxygen in live cells. This work provides a molecular design guideline for further exploring thioketone derivatives as fluorescent probes.