Rapid, Sensitive, Full-Genome Sequencing of Severe Acute Respiratory Syndrome Coronavirus 2
Clinton R. Paden, Ying Tao, Krista Queen, Jing Zhang, Yan Li, Anna Uehara, Suxiang Tong
Abstract
We describe validated protocols for generating high-quality, full-length severe acute respiratory syndrome coronavirus 2 genomes from primary samples. One protocol uses multiplex reverse transcription PCR, followed by MinION or MiSeq sequencing; the other uses singleplex, nested reverse transcription PCR and Sanger sequencing. These protocols enable sensitive virus sequencing in different laboratory environments.
Topics & Concepts
MinionBiologySanger sequencingDNA sequencingGenomeVirologyMultiplexCoronavirusComputational biologyNanopore sequencingGeneticsCoronavirus disease 2019 (COVID-19)MedicineGeneInfectious disease (medical specialty)DiseasePathologySARS-CoV-2 and COVID-19 ResearchAnimal Virus Infections StudiesViral gastroenteritis research and epidemiology