pH-responsive indicator films contained composite anthocyanins for monitoring meat freshness
Zhiyuan Xu, Xizhen Yu, Wenzhe Liu, Jianbo Cheng, Guoyuan Xiong
Abstract
The color changes at different pH (pH 6.0, 7.0 and 8.0) and intermolecular interactions of anthocyanins (purple cabbage (PC), mulberry fruit (MF) and purple sweet potato (PSP)) and their complex with different proportions (10:0, 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8 and 1:9 respectively) were investigated. When PC and MF anthocyanin solutions were mixed in a ratio of 9:1 (PC-MF (9:1)), the color change was most obvious distinguishable at different pH values. According to the fourier transform infrared spectroscopy (FTIR) results, the intermolecular interactions between PC and MF composite anthocyanins were stronger and formed hydrogen bonds. The PC-MF (9:1) indicator film exhibited a distinct color change when chicken was gradually spoiled for 8 d storage at 4 °C. Based on gas chromatography mass spectrometer (GC–MS), the key reason for the color change of indicator film was that the reaction between anthocyanins and gases such as trimethylamine, indole, ammonium carbamate, and butyric acid that can cause pH changes. • Composite anthocyanins from PC and MF were used to monitor chicken spoilage. • Composite anthocyanin of PC:MF = 9:1 had most obvious color change at different pH. • Composite anthocyanins of PC, MF and PSP can be crosslinked by hydrogen bonds. • TMA, Ind, AC and BA from chicken spoilage caused the color change in indicator film. • PC-MF (9:1) film is a handy and visual tool for real-time monitoring meat freshness.