Dynamic full-field optical coherence tomography: 3D live-imaging of retinal organoids
Jules Scholler, Kassandra Groux, Olivier Goureau, José-Alain Sahel, Mathias Fink, Sacha Reichman, Claude Boccara, Kate Grieve
Abstract
Optical coherence tomography offers astounding opportunities to image the complex structure of living tissue but lacks functional information. We present dynamic full-field optical coherence tomography as a technique to noninvasively image living human induced pluripotent stem cell-derived retinal organoids. Coloured images with an endogenous contrast linked to organelle motility are generated, with submicrometre spatial resolution and millisecond temporal resolution, creating a way to identify specific cell types in living tissue via their function.
Topics & Concepts
Optical coherence tomographyCoherence (philosophical gambling strategy)OrganoidRetinalOptical imagingOpticsImage contrastMillisecondComputer scienceHuman eyePhysicsRetinaAdaptive opticsHigh resolutionImage resolutionComputer visionTemporal resolutionPreclinical imagingVisualizationImage processingLiving systemsMicroscopyInduced pluripotent stem cellSuperluminescent diodeBiologyOptical tomographyHigh contrastOptical Coherence Tomography ApplicationsDigital Holography and MicroscopyRandom lasers and scattering media