<i>In Situ</i> Formation of Nanotheranostics to Overcome the Blood–Brain Barrier and Enhance Treatment of Orthotopic Glioma
Haiyan Gao, Chengchao Chu, Yi Cheng, Yang Zhang, Xin Pang, Dengfeng Li, Xiaoyong Wang, En Ren, Fengfei Xie, Yan Bai, Lijuan Chen, Gang Liu, Meiyun Wang
Abstract
Glioblastoma is one of the most lethal cancers and needs effective therapeutics. The development of coordination-driven metal–organic nanoassemblies, which can cross the blood–brain barrier (BBB) and blood–brain tumor barrier (BBTB) and have multiple desired functions, may provide a promising solution to this issue. Here, we report an in situ assembled nanoplatform based on RGD peptide-modified bisulfite-zincII-dipicolylamine-Arg-Gly-Asp (Bis(DPA-Zn)-RGD) and ultrasmall Au-ICG nanoparticles. Attributed to its positive charges and neovascular targeting properties, Bis(DPA-Zn)-RGD can be selectively delivered to the tumor site, and then assembled in situ into large nanoclusters with subsequently administered Au-ICG nanoparticles. Au nanoparticles with ultrasmall size (∼7 nm) can successfully cross the BBB. The obtained nanoclusters exhibit strong near-infrared-red (NIR) absorption and an enhanced tumor retention effect, enabling precise orthotopic fluorescence/photoacoustic imaging. With the aid of image guidance, the photothermal effect of the nanoclusters is observed to suppress tumor progression with the inhibition efficiency reaching up to 93.9%. Meanwhile, no photothermal damage can be found for normal brain tissues. These results, herein, suggest a feasible nanotheranostic agent with the ability to overcome the BBB and BBTB for imaging and therapy of orthotopic brain tumors.