Label-free three-dimensional observations and quantitative characterisation of on-chip vasculogenesis using optical diffraction tomography
Chungha Lee, Seunggyu Kim, Hervé Hugonnet, Moosung Lee, Weisun Park, Jessie S. Jeon, YongKeun Park
Abstract
Label-free, three-dimensional (3D) quantitative observations of on-chip vasculogenesis were achieved using optical diffraction tomography. Exploiting 3D refractive index maps as an intrinsic imaging contrast, the vascular structures, multicellular activities, and subcellular organelles of endothelial cells were imaged and analysed throughout vasculogenesis to characterise mature vascular networks without exogenous labelling.
Topics & Concepts
VasculogenesisDiffractionDiffraction tomographyRefractive indexTomographyOrganelleBiomedical engineeringChipOpticsMaterials scienceChemistryCell biologyBiologyComputer scienceOptoelectronicsEndothelial stem cellPhysicsMedicineBiochemistryIn vitroTelecommunicationsDigital Holography and MicroscopyPhotoacoustic and Ultrasonic ImagingOptical Imaging and Spectroscopy Techniques