Precise Capture and Direct Quantification of Tumor Exosomes <i>via</i> a Highly Efficient Dual-Aptamer Recognition-Assisted Ratiometric Immobilization-Free Electrochemical Strategy
Limin Yang, Xuehan Yin, Bin An, Feng Li
Abstract
value. Consequently, highly sensitive detection of tumor exosomes was achieved. The developed approach successfully realized direct isolation and analysis of tumor exosomes in complex sample media and human serum samples as well. More significantly, this ratiometric dual-signal mode and immobilization-free strategy effectively circumvented the systematic errors caused by external factors and the tedious probe immobilization processes, thus displaying the excellent performances of high reliability, improved accuracy, and easy manipulation. Overall, this approach is expected to offer novel ways for nondestructive early cancer diagnosis.
Topics & Concepts
MicrovesiclesAptamerChemistryDNADual (grammatical number)NanotechnologyMicroemulsionCombinatorial chemistryBiophysicsmicroRNAMolecular biologyBiochemistryMaterials scienceLiteratureBiologyPulmonary surfactantArtGeneExtracellular vesicles in diseaseAdvanced biosensing and bioanalysis techniquesMXene and MAX Phase Materials